Making use of single-molecule optical and AFM imaging and pressure-spectroscopy tactics, we seek out to unravel the mechanistic aspects of the construction and dynamics of chromosomes. Our study pursuits involve:
Reconstitution of ultrawide DNA origami pores in liposomes for transmembrane transportation of macromolecules
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Non-equilibrium folding of unique DNA molecules recaptured as many as one thousand occasions inside a good state nanopore
2010, designed a fresh way (‘wedging transfer’) to govern nanostructures; very first report of DNA translocation as a result of graphene nanopores; and understood hybrid nanopores by directed insertion of α-hemolysin into reliable-point out nanopores
Microbiology has only been around for any century, given that we started to study micro-organisms additional severely. I assumed nanobiology was an acceptable phrase, because all molecular Organic suitable interactions occur at the nanoscale: a protein is a couple of nanometres in dimension, DNA is 2 nm thick.’
All parameters ended up a similar for every panel On this movie, aside from the Original placement of the 6hb rod (shown in blue) around the nanopore. Simulations that terminated early as a result of translocation on the rod from the pore are marked by a gray history.
2011, initial in vitro measurements of transport throughout one biomimetic nuclear pore advanced; development of multiplexed magnetic tweezers for kilo-molecule experiments; and solved the system of homology recognition in DNA homologous recombination
What if, in lieu of employing standard top-down silicon electronics, transistors could possibly be built from The underside up applying solitary organic and natural molecules as the ceesdekker.net switching aspects? This idea, which grew to become The premise of these days’s area of molecular electronics, to start with emerged during the nineteen seventies.
There was one particular dilemma while — we didn't notice any measurable electrical conduction from The one polymer molecules. We concluded that at The one-molecule stage, conducting polymers ended up actually not such fantastic conductors, which was instead disappointing. We Hence broadened our check out to try to discover a lot more promising molecules.
‘The concept that There may be this kind of thing like a starting, a huge bang, is now a fascinating signpost to think about that there was a creator. But far more commonly, and on The premise of pure rationality, I also get there in a Christian interpretation of truth. An atheistic worldview is usually a naturalistic worldview: essentially, you will find only atoms and all-natural forces that we will measure.
‘That considerations the synthetic mobile, as Section of the Dutch BaSyC initiative. Craig Venter seemed best-down in People times and recognized about 5 hundred genes that happen to be minimally essential for a performing cell. We want to search bottom-up: how can we go from a number of molecules to an entire biological program?
Visualization of unstained DNA nanostructures with advanced in-target transmission electron microscopy techniques
Strong-state nanopores have confirmed to become a astonishingly adaptable probe for single-molecule Evaluation of DNA. I will describe some of our current findings – particularly DNA knots – in addition to our efforts to expand the abilities of stable-condition nanopores even even further, during the way of solitary-protein detection, graphene nanopores, plasmonic nanopores, and DNA origami nanopores.